Journal: Microbiology Spectrum

Article Title: Identification and Characterization of Corynaridin, a Novel Linaridin from Corynebacterium lactis

doi: 10.1128/spectrum.01756-22

Figure Lengend Snippet: Antimicrobial activity of purified corynaridin against pathogenic corynebacteria. The RPC fraction was used undiluted and in a dilution series against (a) C. glutamicum ATCC 13032, (b) C. striatum DSM 20668, and (c) C. amycolatum DSM 6922. For each spot, a 10-μL sample was used (RPC fraction with 606 μg/mL total protein). A nisin standard (250 μg/mL) and HPLC-grade H 2 O (control) were used as positive and negative controls, respectively.

Article Snippet: Corynebacterium amycolatum , DSM 6922 , DSMZ.

Techniques: Activity Assay, Purification, Control

Journal: Microbiology Spectrum

Article Title: Identification and Characterization of Corynaridin, a Novel Linaridin from Corynebacterium lactis

doi: 10.1128/spectrum.01756-22

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Corynebacterium amycolatum , DSM 6922 , DSMZ.

Techniques: Plasmid Preparation, Control

Journal: Photochemistry and photobiology

Article Title: UV-induced DNA Damage in Skin is Reduced by CaSR Inhibition.

doi: 10.1111/php.13615

Figure Lengend Snippet: Figure 1. siRNA-mediated reduction in CaSR protein levels reduced UV-induced DNA damage in primary human keratinocytes. (a) Western blot image and densitometry of CaSR densities 48 h post siRNA transfection, at the time of UVR exposure. Expression was normalized to siCtrl to pool data from three independent experiments. a-Tubulin was a loading control. ****P < 0.001 when compared with CaSR protein level under siCtrl condition. DNA damage (b) CPD or (c) 8-OHdG (y-axis) by immunohistochemistry and image analysis in either siCtrl or siCaSR transfected kertainocytes at 3 h post- UV with or without 1,25(OH)2D3 (1,25D). Data were shown as mean SEM (n = 9) relative to SHAM (non-irradiated). ***P < 0.001, **P < 0.01 and *P < 0.05, when compared with siCtrl UV + vehicle; n.s., not significant among treatments. ####P < 0.0001 significantly different from UV + vehi- cle. Photomicrographs of (d) CPD and (e) 8-OHdG in the presence of siCtrl or siCaSR. Black arrows point to the dark brown staining in nuclei indicat- ing the presence of CPD or 8-OHdG. Scale bar = 100 lm.

Article Snippet: CaSR siRNA (siCaSR: sc44373), a pool of three target-specific siRNAs (sequence in Table S1) and scrambled control siRNA (siCtrl: sc-47741) (Santa Cruz).

Techniques: Western Blot, Transfection, Expressing, Control, Immunohistochemistry, Irradiation, Staining